To further understand the distribution of inflammatory cells, particularly lymphoid cells, recruited to the airways and lungs of mice, separate studies were conducted to phenotype the airway cellular influx by flow cytometry see Methods section and Additional file 1 : Figure S1. Assessment of intracellular mucin content in vivo. Human data, not applicable. Although a single exposure to ODE did not impact mucin levels in the airway, it has been demonstrated that repetitive ODE exposure increases Muc5ac expression but that this expression is not at the magnitude seen in experimental allergic asthma models. A review of inflammatory mechanism in airway diseases. Bar graph represents mean with standard error bars shown. ILC2 have been described to breakdown bronchial epithelial barrier integrity [ 40 ], and thus, the increased presence of ILC2 infiltrating the lung tissues of the allergic asthma mice may be important for the predisposition to further disease following ODE exposure. Next, mice were intranasally i.